⁶⁶Ga-PET-imaging of GRPR-expression in prostate cancer: production and characterization of [⁶⁶Ga]Ga-NOTA-PEG₂-RM26.

ABSTRACT: Molecular imaging of the gastrin-releasing peptide receptor (GRPR) could improve patient management in prostate cancer. This study aimed to produce gallium-66 (T_½ = 9.5 h) suitable for radiolabeling, and investigate the imaging properties of gallium-66 labeled GRPR-antagonist NOTA-PEG₂-RM26 for later-time point PET-imaging of GRPR expression. Gallium-66 was cyclotron-produced using a liquid target, and enriched [⁶⁶Zn]Zn(NO₃)₂. In vitro, [⁶⁶Ga]Ga-NOTA-PEG₂-RM26 was characterized in GRPR-expressing PC-3 prostate cancer cells. In vivo, specificity test and biodistribution studies were performed 3 h and 22 h pi in PC-3 xenografted mice. microPET/MR was performed 3 h and 22 h pi. Biodistribution of [⁶⁶Ga]Ga-

SUBMITTER: Rinne SS 

PROVIDER: S-EPMC7878787 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature