Selective loss of microvesicles is a major issue of the differential centrifugation isolation protocols.

ABSTRACT: Microvesicles (MVs) are large extracellular vesicles differing in size, cargo and composition that share a common mechanism of release from the cells through the direct outward budding of the plasma membrane. They are involved in a variety of physiological and pathological conditions and represent promising biomarkers for diseases. MV heterogeneity together with the lack of specific markers had strongly hampered the development of effective methods for MV isolation and differential centrifugation remains the most used method to purify MVs. In this study, we analysed the capacity of the differential centrifugation method to isolate MVs from cell-conditioned medium using flow cytometry and TEM/AFM microscopy. We found that the loss of MVs (general population and/or specific subpopulations) r

SUBMITTER: Nigro A 

PROVIDER: S-EPMC7878808 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature