Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope.

ABSTRACT: The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their dete

SUBMITTER: Trofymchuk K 

PROVIDER: S-EPMC7878865 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature