Three-dimensional vascular microenvironment landscape in human glioblastoma.

ABSTRACT: The cellular complexity of glioblastoma microenvironments is still poorly understood. In-depth, cell-resolution tissue analyses of human material are rare but highly necessary to understand the biology of this deadly tumor. Here we present a unique 3D visualization revealing the cellular composition of human GBM in detail and considering its critical association with the neo-vascular niche. Our images show a complex vascular map of human 3D biopsies with increased vascular heterogeneity and altered spatial relationship with astrocytes or glioma-cell counterparts. High-resolution analysis of the structural layers of the blood brain barrier showed a multilayered fenestration of endothelium and basement membrane. Careful examination of T cell position and migration relative to vascular walls

SUBMITTER: Cribaro GP 

PROVIDER: S-EPMC7879533 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature