Immunological Hallmarks for Clinical Response to BCG in Bladder Cancer.

ABSTRACT: Intravesical Bacillus Calmette-Guerin (BCG) is an effective immunotherapy for non-muscle invasive bladder cancer (NMIBC). However, recurrence and progression remain frequent warranting deeper insights into its mechanism. We herein comprehensively profiled blood and tissues obtained from NMIBC patients before, during and after BCG treatment using cytometry by time-of-flight (CyTOF) and RNA sequencing to identify the key immune subsets crucial for anti-tumor activity. We observed the temporal changes of peripheral immune subsets including NKT cells, central memory CD4⁺ T cells, CD8⁺ T cells and regulatory T cells (Treg) during the course of BCG. Gene expression analysis revealed enriched immune pathways involving in T cell activation and chemotaxis, as well as a more di

SUBMITTER: Lim CJ 

PROVIDER: S-EPMC7879685 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature