Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR.

ABSTRACT: The heat-stable peptidase AprX, secreted by psychrotolerant Pseudomonas species in raw milk, is a major cause of destabilization and premature spoilage of ultra-high temperature (UHT) milk and milk products. To enable rapid detection and quantification of seven frequent and proteolytic Pseudomonas species (P. proteolytica, P. gessardii, P. lactis, P. fluorescens, P. protegens, P. lundensis, and P. fragi) in raw milk, we developed two triplex qPCR assays taking into account species-dependent differences in AprX activity. Besides five species-specific hydrolysis probes, targeting the aprX gene, a universal rpoB probe was included in the assay to determine the total Pseudomonas counts. For all six probes, linear regression lines between C_q value and target DNA concentration were ob

SUBMITTER: Maier C 

PROVIDER: S-EPMC7880948 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature