Enhanced Ca²⁺ influx in mechanically distorted erythrocytes measured with ¹⁹F nuclear magnetic resonance spectroscopy.

ABSTRACT: We present the first direct nuclear magnetic resonance (NMR) evidence of enhanced entry of Ca²⁺ ions into human erythrocytes (red blood cells; RBCs), when these cells are mechanically distorted. For this we loaded the RBCs with the fluorinated Ca²⁺ chelator, 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid (5FBAPTA), and recorded ¹⁹F NMR spectra. The RBCs were suspended in gelatin gel in a special stretching/compression apparatus. The 5FBAPTA was loaded into the cells as the tetraacetoxymethyl ester; and ¹³C NMR spectroscopy with [1,6-¹³C]D-glucose as substrate showed active glycolysis albeit at a reduced rate in cell suspensions and gels. The enhancement of Ca²⁺ influx is concluded to be via the mechanosens

SUBMITTER: Kuchel PW 

PROVIDER: S-EPMC7881017 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature