Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools.

ABSTRACT: Fluorescence-detected linear dichroism microscopy allows observing various molecular processes in living cells, as well as obtaining quantitative information on orientation of fluorescent molecules associated with cellular features. Such information can provide insights into protein structure, aid in development of genetically encoded probes, and allow determinations of lipid membrane properties. However, quantitating and interpreting linear dichroism in biological systems has been laborious and unreliable. Here we present a set of open source ImageJ-based software tools that allow fast and easy linear dichroism visualization and quantitation, as well as extraction of quantitative information on molecular orientations, even in living systems. The tools were tested on model synthetic lipid

SUBMITTER: Bondar A 

PROVIDER: S-EPMC7881160 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature