Temperature modulates immune gene expression in mosquitoes during arbovirus infection.

ABSTRACT: The principal vector of dengue, Zika and chikungunya viruses is the mosquito Aedes aegypti, with its ability to transmit pathogens influenced by ambient temperature. We use chikungunya virus (CHIKV) to understand how the mosquito transcriptome responds to arbovirus infection at different ambient temperatures. We exposed CHIKV-infected mosquitoes to 18, 28 and 32°C, and found that higher temperature correlated with higher virus levels, particularly at 3 days post infection, but lower temperature resulted in reduced virus levels. RNAseq analysis indicated significantly altered gene expression levels in CHIKV infection. The highest number of significantly differentially expressed genes was observed at 28°C, with a more muted effect at the other temperatures. At the higher temperature,

SUBMITTER: Wimalasiri-Yapa BMCR 

PROVIDER: S-EPMC7881175 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature