Fibronectin mediates activation of stromal fibroblasts by SPARC in endometrial cancer cells.

ABSTRACT:

Background

Matricellular glycoprotein, SPARC is a secreted molecule, that mediates the interaction between cells and extracellular matrix. SPARC functions as a regulator of matrix organization and modulates cell behavior. In various kinds of cancer, strong SPARC expression was observed in stromal tissues as well as in cancer epithelial cells. The function of SPARC in cancer cells is somewhat controversial and its impact on peritumoral stromal cells remains to be resolved.

Methods

We investigated the effects of SPARC expression in endometrial cancer cells on the surrounding stromal fibroblasts using in vitro co-culture system. Changes in characteristics of fibroblasts were examined by analysis of fibroblast-specific markers and in vitro contraction assay.

Results

SPARC

SUBMITTER: Yoshida S 

PROVIDER: S-EPMC7881467 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature