Isolation, culture, and characterization of chicken intestinal epithelial cells.

ABSTRACT:

Background

Enterocytes exert an absorptive and protective function in the intestine, and they encounter many different challenging factors such as feed, bacteria, and parasites. An intestinal epithelial in vitro model can help to understand how enterocytes are affected by these factors and contribute to the development of strategies against pathogens.

Results

The present study describes a novel method to culture and maintain primary chicken enterocytes and their characterization by immunofluorescence and biomolecular approaches. Starting from 19-day-old chicken embryos it was possible to isolate viable intestinal cell aggregates that can expand and produce a self-maintaining intestinal epithelial cell population that survives until 12 days in culture. These cells resulted po

SUBMITTER: Ghiselli F 

PROVIDER: S-EPMC7881477 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature