Novel fusion peptide-mediated siRNA delivery using self-assembled nanocomplex.

ABSTRACT:

Background

Gene silencing using siRNA can be a new potent strategy to treat many incurable diseases at the genetic level, including cancer and viral infections. Treatments using siRNA essentially requires an efficient and safe method of delivering siRNA into cells while maintaining its stability. Thus, we designed novel synergistic fusion peptides, i.e., SPACE and oligoarginine.

Results

Among the novel fusion peptides and siRNAs, nanocomplexes have enhanced cellular uptake and gene silencing effect in vitro and improved retention and gene silencing effects of siRNAs in vivo. Oligoarginine could attract siRNAs electrostatically to form stable and self-assembled nanocomplexes, and the SPACE peptide could interact with the cellular membrane via hydrogen bonding. Therefore, nano

SUBMITTER: Ryu YC 

PROVIDER: S-EPMC7881583 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature