Inducing secondary metabolite production of Aspergillus sydowii through microbial co-culture with Bacillus subtilis.

ABSTRACT:

Background

The co-culture strategy which mimics natural ecology by constructing an artificial microbial community is a useful tool to activate the biosynthetic gene clusters to generate new metabolites. However, the conventional method to study the co-culture is to isolate and purify compounds separated by HPLC, which is inefficient and time-consuming. Furthermore, the overall changes in the metabolite profile cannot be well characterized.

Results

A new approach which integrates computational programs, MS-DIAL, MS-FINDER and web-based tools including GNPS and MetaboAnalyst, was developed to analyze and identify the metabolites of the co-culture of Aspergillus sydowii and Bacillus subtilis. A total of 25 newly biosynthesized metabolites were detected only in co-culture. The s

SUBMITTER: Sun Y 

PROVIDER: S-EPMC7881642 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature