Functional identification of an opsin kinase underlying inactivation of the pineal bistable opsin parapinopsin in zebrafish.

ABSTRACT: In the pineal organ of zebrafish larvae, the bistable opsin parapinopsin alone generates color opponency between UV and visible light. Our previous study suggested that dark inactivation of the parapinopsin photoproduct, which activates G-proteins, is important for the regulation of the amount of the photoproduct. In turn, the photoproduct is responsible for visible light sensitivity in color opponency. Here, we found that an opsin kinase or a G-protein-coupled receptor kinase (GRK) is involved in inactivation of the active photoproduct of parapinopsin in the pineal photoreceptor cells of zebrafish larvae. We investigated inactivation of the photoproduct in the parapinopsin cells of various knockdown larvae by measuring the light responses of the cells using calcium imaging. We found that

SUBMITTER: Shen B 

PROVIDER: S-EPMC7881645 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature