Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients

ABSTRACT: Abstract

Introduction

Disruption of copper homeostasis and dysfunction of mitochondria have been documented in sclerotic hippocampi (HS) of patients with mesial temporal lobe epilepsy (mTLE). However, a potential link between these pathological changes has not been tackled so far. Herein, we analyzed regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase (CCO), a component of mitochondrial electron transport chain and copper‐containing metalloprotein, in HS.

Methods

Histochemical staining and laser ablation inductively coupled plasma mass spectrometry were carried out to construct comparable maps of these parameters in coronal sections of hippocampi of 3 mTLE‐HS patients and 3 control subjects.

Results

Coppe

SUBMITTER: Opacic M 

PROVIDER: S-EPMC7882178 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature