Role of 25-Hydroxyvitamin D₃ and 1,25-Dihydroxyvitamin D₃ in Chicken Embryo Osteogenesis, Adipogenesis, Myogenesis, and Vitamin D₃ Metabolism.

ABSTRACT: A study was conducted to understand the effects of 25-hydroxyvitamin D₃ (25OHD) and 1,25-dihydroxyvitamin D₃ (1,25OHD) administration on the expression of key genes related to osteogenesis, adipogenesis, myogenesis, and vitamin D₃ metabolism in the chicken embryo. A total of 120 fertilized Cobb 500 eggs were used in the current study and were reared under standard incubation conditions. On embryonic day 3 (ED 3), PBS (C), PBS with 40ng 1,25OHD (1,25D-L), 200ng 1,25OHD (1,25D-H), 40ng 25OHD (25D-L), or 200ng 25OHD (25D-H) were injected into the dorsal vein of developing embryos. Whole embryos were harvested at 1, 3, and 6h post-injection for gene expression analyses (n=8). Gene expression for key osteogenesis markers (RUNX2: runt-related transc

SUBMITTER: Chen C 

PROVIDER: S-EPMC7882680 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature