Biosurveillance for invasive insect pest species using an environmental DNA metabarcoding approach and a high salt trap collection fluid.

ABSTRACT: With the increase in global trade and warming patterns, the movement, introduction, and establishment of non-native insect species has increased. A rapid and effective early detection biosurveillance program to identify species of concern is needed to reduce future impacts and costs associated with introduced non-native species. One of the challenges facing insect surveillance trapping methods is the sheer volume of individual specimens in the collections. Although molecular identification methods are improving, they currently have limitations (e.g., destructive processing of specimens) and a protocol addressing these limitations can support regulatory applications that need morphological evidence to corroborate molecular data.The novel protocol presented here uses a metabarcoding approach

SUBMITTER: Young RG 

PROVIDER: S-EPMC7882945 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature