Dataset Information

Best practices for the visualization, mapping, and manipulation of R-loops.

ABSTRACT: R-loops represent an abundant class of large non-B DNA structures in genomes. Even though they form transiently and at modest frequencies, interfering with R-loop formation or dissolution has significant impacts on genome stability. Addressing the mechanism(s) of R-loop-mediated genome destabilization requires a precise characterization of their distribution in genomes. A number of independent methods have been developed to visualize and map R-loops, but their results are at times discordant, leading to confusion. Here, we review the main existing methodologies for R-loop mapping and assess their limitations as well as the robustness of existing datasets. We offer a set of best practices to improve the reproducibility of maps, hoping that such guidelines could be useful for authors and referees alike. Finally, we propose a possible resolution for the apparent contradictions in R-loop mapping outcomes between antibody-based and RNase H1-based mapping approaches.

SUBMITTER: Chedin F

PROVIDER: S-EPMC7883053 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Best practices for the visualization, mapping, and manipulation of R-loops.

Chédin Frédéric F Hartono Stella R SR Sanz Lionel A LA Vanoosthuyse Vincent V

The EMBO journal 20210107 4

R-loops represent an abundant class of large non-B DNA structures in genomes. Even though they form transiently and at modest frequencies, interfering with R-loop formation or dissolution has significant impacts on genome stability. Addressing the mechanism(s) of R-loop-mediated genome destabilization requires a precise characterization of their distribution in genomes. A number of independent methods have been developed to visualize and map R-loops, but their results are at times discordant, le ...[more]

PMID: 33411340

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Dataset Information

Best practices for the visualization, mapping, and manipulation of R-loops.

Publications

Best practices for the visualization, mapping, and manipulation of R-loops.

Similar Datasets

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