Crystal structure of bacterial cytotoxic necrotizing factor CNF_Y reveals molecular building blocks for intoxication.

ABSTRACT: Cytotoxic necrotizing factors (CNFs) are bacterial single-chain exotoxins that modulate cytokinetic/oncogenic and inflammatory processes through activation of host cell Rho GTPases. To achieve this, they are secreted, bind surface receptors to induce endocytosis and translocate a catalytic unit into the cytosol to intoxicate host cells. A three-dimensional structure that provides insight into the underlying mechanisms is still lacking. Here, we determined the crystal structure of full-length Yersinia pseudotuberculosis CNF_Y . CNF_Y consists of five domains (D1-D5), and by integrating structural and functional data, we demonstrate that D1-3 act as export and translocation module for the catalytic unit (D4-5) and for a fused β-lactamase reporter protein. We further found

SUBMITTER: Chaoprasid P 

PROVIDER: S-EPMC7883292 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature