Activity-Based Protein Profiling of Retaining α-Amylases in Complex Biological Samples.

ABSTRACT: Amylases are key enzymes in the processing of starch in many kingdoms of life. They are important catalysts in industrial biotechnology where they are applied in, among others, food processing and the production of detergents. In man amylases are the first enzymes in the digestion of starch to glucose and arguably also the preferred target in therapeutic strategies aimed at the treatment of type 2 diabetes patients through down-tuning glucose assimilation. Efficient and sensitive assays that report selectively on retaining amylase activities irrespective of the nature and complexity of the biomaterial studied are of great value both in finding new and effective human amylase inhibitors and in the discovery of new microbial amylases with potentially advantageous features for biotechnologica

SUBMITTER: Chen Y 

PROVIDER: S-EPMC7883350 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature