Increase of aerobic glycolysis mediated by activated T helper cells drives synovial fibroblasts towards an inflammatory phenotype: new targets for therapy?

ABSTRACT:

Background

A dysregulated glucose metabolism in synovial fibroblasts (SF) has been associated with their aggressive phenotype in rheumatoid arthritis (RA). Even though T helper (Th) cells are key effector cells in the propagation and exacerbation of synovitis in RA, little is known about their influence on the metabolism of SF. Thus, this study investigates the effect of Th cells on the glucose metabolism and phenotype of SF and how this is influenced by the blockade of cytokines, janus kinases (JAKs) and glycolysis.

Methods

SF from patients with RA or osteoarthritis (OA) were cultured in the presence of a stable glucose isotopomer ([U-¹³C]-glucose) and stimulated with the conditioned media of activated Th cells (ThCM). Glucose consumption and lactate production w

SUBMITTER: Kvacskay P 

PROVIDER: S-EPMC7883459 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature