Bacillus phage endolysin, lys46, bactericidal properties against Gram-negative bacteria.

ABSTRACT:

Background and objectives

The great potential of bacteriophage for removing pathogen bacteria via targeting the cell wall is highly concerned. With a priority for overcoming drug-resistance, we screened against endolysins targeting Gram-negative bacteria to introduce a new antibacterial agent. This study was aimed to identify endolysins from the lysogenic phage of the Siphoviridea family in Bacillus subtilis.

Materials and methods

The Bacillus subtilis strain DDBCC46 was isolated from a preliminary antibacterial screening program. The endolysin (s) was extracted, concentrated with ammonium sulfate saturation, and their activity evaluated against the indicator bacteria. The phage particles were extracted from the bacteria using the minimum inhibition conc

SUBMITTER: Sarjoughian MR 

PROVIDER: S-EPMC7884283 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature