Ocular Surface Microbiome Alterations Are Found in Both Eyes of Individuals With Unilateral Infectious Keratitis.

ABSTRACT:

Purpose

To analyze the ocular surface microbiome (OSM) profile in both eyes of individuals with unilateral keratitis.

Methods

In this prospective, cross-sectional study, the conjunctival OSM of adults (>18 years old) presenting to an ophthalmic emergency department with acute unilateral keratitis and controls without an acute infectious process was sampled. Samples underwent DNA amplification and 16S sequencing using Illumina MiSeq 250 and were analyzed using Qiime. Statistical analysis was performed using a two-sided Student t-test, diversity indices, and principal coordinate analysis. The main outcome measures included relative abundance and α and β diversity.

Results

Bacterial DNA was recovered from all 34 eyes of 17 individuals with keratitis (mean age, 49.3 ± 17.

SUBMITTER: Cavuoto KM 

PROVIDER: S-EPMC7884290 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature