Identification and validation of suitable reference genes for quantitative real-time PCR gene expression analysis in pregnant human myometrium.

ABSTRACT: Accurate quantification of quantitative PCR (qPCR) data requires a set of stable reference genes (RGs) for normalisation. Despite its importance to mechanistic studies, no evaluation of RG stability has been conducted for pregnant human myometrium. A systematic search of the literature was performed to identify the most used RGs in human myometrial gene expression studies. The stability of these genes, and others, was then evaluated using geNorm and NormFinder algorithms, in samples of myometrium from singleton or twin pregnancies (n = 7 per group) delivering at term or preterm. The most frequently cited RGs were GAPDH, ACTB, B2M and 18s. There was strong agreement between algorithms on the most and least stable genes: Both indicated CYC1, YWHAZ and ATP5B were the most stably expressed. De

SUBMITTER: Arrowsmith S 

PROVIDER: S-EPMC7884357 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature