Single-Cell RNA Sequencing Reveals the Expansion of Cytotoxic CD4⁺ T Lymphocytes and a Landscape of Immune Cells in Primary Sjogren's Syndrome.

ABSTRACT:

Objective

Primary Sjögren's syndrome (pSS) is a systemic autoimmune disease, and its pathogenetic mechanism is far from being understood. In this study, we aimed to explore the cellular and molecular mechanisms that lead to pathogenesis of this disease.

Methods

We applied single-cell RNA sequencing (scRNA-seq) to 57,288 peripheral blood mononuclear cells (PBMCs) from five patients with pSS and five healthy controls. The immune cell subsets and susceptibility genes involved in the pathogenesis of pSS were analyzed. Flow cytometry was preformed to verify the result of scRNA-seq.

Results

We identified two subpopulations significantly expand in pSS patients. The one highly expressing cytotoxicity genes is named as CD4⁺ CTLs cytotoxic T lymphocyte, and another h

SUBMITTER: Hong X 

PROVIDER: S-EPMC7884617 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature