Microbiome-based environmental monitoring of a dairy processing facility highlights the challenges associated with low microbial-load samples.

ABSTRACT: Efficient and accurate identification of microorganisms throughout the food chain can potentially allow the identification of sources of contamination and the timely implementation of control measures. High throughput DNA sequencing represents a potential means through which microbial monitoring can be enhanced. While Illumina sequencing platforms are most typically used, newer portable platforms, such as the Oxford Nanopore Technologies (ONT) MinION, offer the potential for rapid analysis of food chain microbiomes. Initial assessment of the ability of rapid MinION-based sequencing to identify microbes within a simple mock metagenomic mixture is performed. Subsequently, we compare the performance of both ONT and Illumina sequencing for environmental monitoring of an active food processing

SUBMITTER: McHugh AJ 

PROVIDER: S-EPMC7884712 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature