One-tube SARS-CoV-2 detection platform based on RT-RPA and CRISPR/Cas12a.

ABSTRACT:

Background

COVID-19 has spread rapidly around the world, affecting a large percentage of the population. When lifting certain mandatory measures for an economic restart, robust surveillance must be established and implemented, with nucleic acid detection for SARS-CoV-2 as an essential component.

Methods

We tried to develop a one-tube detection platform based on RT-RPA (Reverse Transcription and Recombinase Polymerase Isothermal Amplification) and DNA Endonuclease-Targeted CRISPR Trans Reporter (DETECTR) technology, termed OR-DETECTR, to detect SARS-CoV-2. We designed RT-RPA primers of the RdRp and N genes following the SARS-CoV-2 gene sequence. We optimized reaction components so that the detection process could be carried out in one tube. Specificity was demonstrated by det

SUBMITTER: Sun Y 

PROVIDER: S-EPMC7884969 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature