Characterizing RNA stability genome-wide through combined analysis of PRO-seq and RNA-seq data.

ABSTRACT:

Background

The concentrations of distinct types of RNA in cells result from a dynamic equilibrium between RNA synthesis and decay. Despite the critical importance of RNA decay rates, current approaches for measuring them are generally labor-intensive, limited in sensitivity, and/or disruptive to normal cellular processes. Here, we introduce a simple method for estimating relative RNA half-lives that is based on two standard and widely available high-throughput assays: Precision Run-On sequencing (PRO-seq) and RNA sequencing (RNA-seq).

Results

Our method treats PRO-seq as a measure of transcription rate and RNA-seq as a measure of RNA concentration, and estimates the rate of RNA decay required for a steady-state equilibrium. We show that this approach can be used to assay rel

SUBMITTER: Blumberg A 

PROVIDER: S-EPMC7885420 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature