Mfd Affects Global Transcription and the Physiology of Stressed Bacillus subtilis Cells.

ABSTRACT: For several decades, Mfd has been studied as the bacterial transcription-coupled repair factor. However, recent observations indicate that this factor influences cell functions beyond DNA repair. Our lab recently described a role for Mfd in disulfide stress that was independent of its function in nucleotide excision repair and base excision repair. Because reports showed that Mfd influenced transcription of single genes, we investigated the global differences in transcription in wild-type and mfd mutant growth-limited cells in the presence and absence of diamide. Surprisingly, we found 1,997 genes differentially expressed in Mfd^- cells in the absence of diamide. Using gene knockouts, we investigated the effect of genetic interactions between Mfd and the genes in its regul

SUBMITTER: Martin HA 

PROVIDER: S-EPMC7885715 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature