A rapid α-synuclein seed assay of Parkinson's disease CSF panel shows high diagnostic accuracy.

ABSTRACT:

Background

Assays that specifically measure α-synuclein seeding activity in biological fluids could revolutionize the diagnosis of Parkinson's disease. Recent improvements in α-synuclein real-time quaking-induced conversion assays of cerebrospinal fluid have dramatically reduced reaction times from 5-13 days down to 1-2 days.

Objective

To test our improved assay against a panel of cerebrospinal fluid specimens from patients with Parkinson's disease and healthy controls from the MJ Fox Foundation/NINDS BioFIND collection.

Methods

Specimens collected from healthy controls and patients with clinically typical moderate-to-advanced Parkinson's disease were tested without prior knowledge of disease status. Correlative analyses between assay parameters and clinical measures

SUBMITTER: Orru CD 

PROVIDER: S-EPMC7886040 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature