Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection.

ABSTRACT:

Background

Two automatable in-house protocols for high-troughput RNA extraction from nasopharyngeal swabs for SARS-CoV-2 detection have been evaluated.

Methods

One hundred forty one SARS-CoV-2 positive samples were collected during a period of 10-days. In-house protocols were based on extraction with magnetic beads and designed to be used with either the Opentrons OT-2 (OT-2in-house) liquid handling robot or the MagMAXTM Express-96 system (MMin-house). Both protocols were tested in parallel with a commercial kit that uses the MagMAXTM system (MMkit). Nucleic acid extraction efficiencies were calculated from a SARS-CoV-2 DNA positive control.

Results

No significant differences were found between both in-house protocols and the commercial kit in their performance to det

SUBMITTER: Lazaro-Perona F 

PROVIDER: S-EPMC7886139 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature