BipA exerts temperature-dependent translational control of biofilm-associated colony morphology in Vibrio cholerae.

ABSTRACT: Adaptation to shifting temperatures is crucial for the survival of the bacterial pathogen Vibrio cholerae. Here, we show that colony rugosity, a biofilm-associated phenotype, is regulated by temperature in V. cholerae strains that naturally lack the master biofilm transcriptional regulator HapR. Using transposon-insertion mutagenesis, we found the V. cholerae ortholog of BipA, a conserved ribosome-associated GTPase, is critical for this temperature-dependent phenomenon. Proteomic analyses revealed that loss of BipA alters the synthesis of >300 proteins in V. cholerae at 22°C, increasing the production of biofilm-related proteins including the key transcriptional activators VpsR and VpsT, as well as proteins important for diverse cellular processes. At low temper

SUBMITTER: Del Peso Santos T 

PROVIDER: S-EPMC7886329 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature