Schistosoma japonicum Infection in Treg-Specific USP21 Knockout Mice.

ABSTRACT: The E3 deubiquitinating enzyme ubiquitin-specific proteolytic enzyme 21 (USP21) plays vital roles in physiological activities and is required for Treg-cell-mediated immune tolerance. Using a murine model infected with Schistosoma japonicum, we observed that there were more cercariae developed into adults and more eggs deposited in the livers of the USP21^fl/flFOXP3^Cre (KO) mice. However, immunohistochemistry showed that the degree of egg granuloma formation and liver fibrosis was reduced. In USP21^fl/flFOXP3^Cre mice, levels of IFN-gamma, IL-4, anti-soluble egg antigen (SEA) IgG and anti-soluble worm antigen preparation (SWAP) IgG increased in blood, as determined using ELISAs and multiplex fluorescent microsphere immunoassays, while the leve

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7886505 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature