Liver damage favors the eliminations of HBV integration and clonal hepatocytes in chronic hepatitis B.

ABSTRACT:

Background

HBV integration is suspected to be an obstinate risk factor for hepatocellular carcinoma (HCC) in the era of antiviral therapy. Integration events start to occur in the immunotolerance phase, but their fates in the immune clearance phase have not yet been clarified. Here, we report the influences of liver damage on HBV integration and clonal hepatocyte expansion in patients with chronic hepatitis B (CHB).

Methods

HBV integration breakpoints in liver biopsy samples from 54 CHB patients were detected using a modified next-generation sequencing assay.

Results

A total of 3729 (69 per sample) integration breakpoints were found in the human genome, including some hotspot genes and KEGG pathways, especially in patients with abnormal transaminases. The number of br

SUBMITTER: Hu G 

PROVIDER: S-EPMC7886763 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature