Transcriptomic Identification of Draxin-Responsive Targets During Cranial Neural Crest EMT.

ABSTRACT: Canonical Wnt signaling plays an essential role in proper craniofacial morphogenesis, at least partially due to regulation of various aspects of cranial neural crest development. In an effort to gain insight into the etiology of craniofacial abnormalities resulting from Wnt signaling and/or cranial neural crest dysfunction, we sought to identify Wnt-responsive targets during chick cranial neural crest development. To this end, we leveraged overexpression of a canonical Wnt antagonist, Draxin, in conjunction with RNA-sequencing of cranial neural crest cells that have just activated their epithelial-mesenchymal transition (EMT) program. Through differential expression analysis, gene list functional annotation, hybridization chain reaction (HCR), and quantitative reverse transcription polymer

SUBMITTER: Hutchins EJ 

PROVIDER: S-EPMC7886793 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature