Accurate, Efficient and User-Friendly Mutation Calling and Sample Identification for TILLING Experiments.

ABSTRACT: TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful reverse genetics method in plant functional genomics and breeding to identify mutagenized individuals with improved behavior for a trait of interest. Pooled high throughput sequencing (HTS) of the targeted genes allows efficient identification and sample assignment of variants within genes of interest in hundreds of individuals. Although TILLING has been used successfully in different crops and even applied to natural populations, one of the main issues for a successful TILLING experiment is that most currently available bioinformatics tools for variant detection are not designed to identify mutations with low frequencies in pooled samples or to perform sample identification from variants identified in overlapping pools. Ou

SUBMITTER: Gil J 

PROVIDER: S-EPMC7886796 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature