⁸⁹Zr anti-CD44 immuno-PET monitors  CD44 expression on splenic myeloid cells and HT29 colon cancer cells.

ABSTRACT: CD44 is a cell-surface glycoprotein involved in cell-cell interaction, adhesion, and migration. CD44 is found on colon cancer cells and on immune cells. Previous studies of ⁸⁹Zr PET imaging of CD44 have relied on an anti-human antibody (Ab), which can influence biodistribution in murine models. In this study, we used an Ab that cross-reacts with both human and mouse origin CD44 of all isoforms to unveil the type of leukocyte responsible for high splenic anti-CD44 uptake and investigate how its regulation can influence tumor immuno-PET. The Ab was site-specifically labeled with ⁸⁹Zr-deferoxamine on cysteine residues. ⁸⁹Zr-anti-CD44 demonstrated high-specific binding to HT29 human colon cancer cells and monocytic cells that showed CD44 expression. When ⁸

SUBMITTER: Park JW 

PROVIDER: S-EPMC7887231 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature