Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3.

ABSTRACT: Amino acid substitutions within the conserved polypeptide sequence of the insect olfactory receptor co-receptor (Orco) have been demonstrated to influence its pharmacological properties. By sequence analysis and phylogenetic investigation, in the Lepidopteran subgroup Ditrysia we identified a fixed substitution in the intracellular loop-3 (ICL-3) of a conserved histidine to glutamine. By means of HEK293 cells as a heterologous system, we functionally expressed Orco from the Ditrysian model Cydia pomonella (CpomOrco) and compared its functional properties with a site-directed mutagenized version where this ICL-3-glutamine was reverted to histidine (CpomOrco^Q417H). The mutagenized CpomOrco^Q417H displayed decreased responsiveness to VUAA1 and reduced response efficacy to

SUBMITTER: Bobkov YV 

PROVIDER: S-EPMC7887336 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature