Ontology highlight
ABSTRACT:
SUBMITTER: Casadei L
PROVIDER: S-EPMC7887429 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
Casadei Lucia L Choudhury Adarsh A Sarchet Patricia P Mohana Sundaram Prashanth P Lopez Gonzalo G Braggio Danielle D Balakirsky Gita G Pollock Raphael R Prakash Shaurya S
Journal of extracellular vesicles 20210216 4
We present a resource-efficient approach to fabricate and operate a micro-nanofluidic device that uses cross-flow filtration to isolate and capture liposarcoma derived extracellular vesicles (EVs). The isolated extracellular vesicles were captured using EV-specific protein markers to obtain vesicle enriched media, which was then eluted for further analysis. Therefore, the micro-nanofluidic device integrates the unit operations of size-based separation with CD63 antibody immunoaffinity-based capt ...[more]