Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to Wistar rats.

ABSTRACT: Aromatase, an estrogen synthase, exists in the gastric parietal cells of Wistar rats. The stomach synthesizes large amounts of estrogens and secretes them into the portal vein. We have been particularly studying gastric estrogen synthesis using Wistar rats. However, estrogen synthesis in the stomach of Sprague-Dawley (SD) rats, which are used as frequently as those of the Wistar strain, has not been clarified. We examined steroid synthesis in the stomach of SD rats using immunohistochemistry, in situ hybridization, Western blotting, real-time PCR, and LC-MS/MS. Aromatase also exists in the stomach of SD rats. Its distribution was not found to be different from that of Wistar rats. Results show that H⁺/K⁺-ATPase β-subunit and aromatase colocalized in double immunofluor

SUBMITTER: Kobayashi H 

PROVIDER: S-EPMC7887622 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature