Acute Lymph Node Slices Are a Functional Model System to Study Immunity Ex Vivo.

ABSTRACT: The lymph node is a highly organized and dynamic structure that is critical for facilitating the intercellular interactions that constitute adaptive immunity. Most ex vivo studies of the lymph node begin by reducing it to a cell suspension, thus losing the spatial organization, or fixing it, thus losing the ability to make repeated measurements. Live murine lymph node tissue slices offer the potential to retain spatial complexity and dynamic accessibility, but their viability, level of immune activation, and retention of antigen-specific functions have not been validated. Here we systematically characterized live murine lymph node slices as a platform to study immunity. Live lymph node slices maintained the expected spatial organization and cell populations while reflecting the 3D spatial

SUBMITTER: Belanger MC 

PROVIDER: S-EPMC7887751 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature