HilE is required for synergistic activation of SPI-1 gene expression in Salmonella enterica serovar Typhimurium.

ABSTRACT:

Background

Salmonella enterica serovar Typhimurium is an intestinal pathogen capable of infecting a wide range of animals. It initiates infection by invading intestinal epithelial cells using a type III secretion system encoded within Salmonella pathogenicity island 1 (SPI-1). The SPI-1 genes are regulated by multiple interacting transcription factors. The master regulator is HilD. HilE represses SPI-1 gene expression by binding HilD and preventing it from activating its target promoters. Previous work found that acetate and nutrients synergistically induce SPI-1 gene expression. In the present study, we investigated the role of HilE, nominally a repressor of SPI-1 gene expression, in mediating this response to acetate and nutrients.

Results

HilE is necessary for activation

SUBMITTER: Hamed S 

PROVIDER: S-EPMC7887791 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature