Assessment of pregnancy-associated glycoprotein profile in milk for early pregnancy diagnosis in goats.

ABSTRACT:

Objective

This study was conducted to assess the level of pregnancy-associated glycoprotein (PAG) in whole and skim milk samples, and its suitability for early pregnancy diagnosis in goats.

Methods

A two-step sandwich enzyme-linked immunosorbent assay (ELISA) system for estimation of milk PAG was developed and validated, which employed caprine-PAG specific polyclonal antisera. Whole and skim milk samples (n = 210 each) from fifteen multiparous goats were collected on alternate days from d 10 to d 30, and thereafter weekly till d 51 postmating. PAG levels in milk samples were estimated by ELISA and the pregnancies were confirmed at d40 post-mating by transrectal ultrasonography (TRUS).

Results

The level of PAG in whole and skim milk samples of both pregnant and nonpreg

SUBMITTER: Singh SP 

PROVIDER: S-EPMC7888492 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature