Effect of JAK-STAT pathway in regulation of fatty liver hemorrhagic syndrome in chickens.

ABSTRACT:

Objective

To explore the molecular mechanisms of fatty liver hemorrhagic syndrome (FLHS) in laying hens, an experiment was conducted to reveal the differences in histopathological observation and gene expression between FLHS group and normal group.

Methods

We compared the histopathological difference using hematoxylin and eosin staining and proceeded with RNA sequencing of adipose tissue to search differentially expressed genes and enriched biological processes and pathways. Then we validated the mRNA expression levels by real-time polymerase chain reaction and quantified protein levels in the circulation by enzyme-linked immunosorbent assay.

Results

We identified 100 differentially expressed transcripts corresponding to 66 genes (DEGs) were identified between FLHS-af

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC7888496 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature