Validation of exercise-response genes in skeletal muscle cells of Thoroughbred racing horses.

ABSTRACT:

Objective

To understand the athletic characteristics of Thoroughbreds, high-throughput analysis has been conducted using horse muscle tissue. However, an in vitro system has been lacking for studying and validating genes from in silico data. The aim of this study is to validate genes from differentially expressed genes (DEGs) of our previous RNA-sequencing data in vitro. Also, we investigated the effects of exercise-induced stress including heat, oxidative, hypoxic and cortisol stress on horse skeletal muscle derived cells with the top six upregulated genes of DEGs.

Methods

Enriched pathway analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) tool with upregulated genes in horse skeletal muscle tissue after exercise. Among

SUBMITTER: Kim DH 

PROVIDER: S-EPMC7888507 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature