Copper induces transcription of BcLCC2 laccase gene in phytopathogenic fungus, Botrytis cinerea.

ABSTRACT: Laccases are one of many groups of inducible enzymes produced by the filamentous fungus, Botrytis cinerea during colonisation of host plant tissues. While the processes involved in laccase induction are not fully understood, Cupric ions (e.g. CuSO₄) and gallic acid (GA) have been reported as laccase inducers. This study investigates laccases activities and the expression of three laccase genes (BcLCC1, BcLCC2, BcLCC3) in three B. cinerea isolates grown in laccase-inducing medium (LIM) supplemented with CuSO₄ and GA. Laccase activity in culture filtrates with CuSO₄ increased after 48 h of growth in LIM at 24°C. The induction of BcLCC2 transcription was greatest at a concentration of 0.6 mM CuSO₄, concentrations greater

SUBMITTER: Buddhika UVA 

PROVIDER: S-EPMC7889114 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature