Anti-Ly6G binding and trafficking mediate positive neutrophil selection to unleash the anti-tumor efficacy of radiation therapy.

ABSTRACT: The anti-Ly6G antibody is used to deplete Ly6G^pos neutrophils and study their role in diverse pathologies. However, depletion is never absolute, as Ly6G^low neutrophils resistant to depletion rapidly emerge. Studying the functionality of these residual neutrophils is necessary to interpret anti-Ly6G-based experimental designs. In vitro, we found anti-Ly6G binding induced Ly6G internalization, surface Ly6G paucity, and primed the oxidative burst of neutrophils upon TNF α co-stimulation. In vivo, we found neutrophils resistant to anti-Ly6G depletion exhibited anti-neutrophil-cytoplasmic-antibodies. In the pre-clinical Kras^{Lox-STOP-Lox-G12D/WT}; Trp53^Flox/Flox mouse lung tumor model, abnormal neutrophil accumulation and aging was

SUBMITTER: Boivin G 

PROVIDER: S-EPMC7889163 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature