Dataset Information

The complete chloroplast genome of Celtis julianae (Ulmaceae).

ABSTRACT: Celtis julianae C.K. Schneid. is a large deciduous tree of Ulmaceae. In this study, the chloroplast genome sequence of C. julianae was 159,064 bp in length, consisting of a large single-copy (LSC) region with 86,139 bp, a small single-copy (SSC) region with 19,137 bp, and two inverted repeat regions (IRs) with 26,894 bp. The GC content in the chloroplast genome of C. julianae was 36.3%. The chloroplast genome of C. julianae contained 127 genes, including 86 protein-coding genes, 37 tRNA genes, and four rRNA genes. Phylogenetic tree showed that C. julianae was clustered with C. tetrandra.

SUBMITTER: Wang X

PROVIDER: S-EPMC7889233 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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The complete chloroplast genome of Celtis julianae (Ulmaceae).

Wang Xudong X Liu Huadong H Jing Sen S Liu Yuhong Y

Mitochondrial DNA. Part B, Resources 20210211 2

Celtis julianae C.K. Schneid. is a large deciduous tree of Ulmaceae. In this study, the chloroplast genome sequence of C. julianae was 159,064 bp in length, consisting of a large single-copy (LSC) region with 86,139 bp, a small single-copy (SSC) region with 19,137 bp, and two inverted repeat regions (IRs) with 26,894 bp. The GC content in the chloroplast genome of C. julianae was 36.3%. The chloroplast genome of C. julianae contained 127 genes, including 86 protein-co ...[more]

PMID: 33628910

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Project description:In this study, the chloroplast (cp) genomes of Hemiptelea davidii, Ulmus parvifolia, Ulmus lamellosa, Ulmus castaneifolia, and Ulmus pumila 'zhonghuajinye' were spliced, assembled and annotated using the Illumina HiSeq PE150 sequencing platform, and then compared to the cp genomes of other Ulmus and Ulmaceae species. The results indicated that the cp genomes of the five sequenced species showed a typical tetrad structure with full lengths ranging from 159,113 to 160,388 bp. The large single copy (LSC), inverted repeat (IR), and small single copy (SSC) lengths were in the range of 87,736-88,466 bp, 26,317-26,622 bp and 18,485-19,024 bp, respectively. A total of 130-131 genes were annotated, including 85-86 protein-coding genes, 37 tRNA genes and eight rRNA genes. The GC contents of the five species were similar, ranging from 35.30 to 35.62%. Besides, the GC content was different in different region and the GC content in IR region was the highest. A total of 64-133 single sequence repeat (SSR) loci were identified among all 21 Ulmaceae species. The (A)n and (T)n types of mononucleotide were highest in number, and the lengths were primarily distributed in 10-12 bp, with a clear AT preference. A branch-site model and a Bayes Empirical Bayes analysis indicated that the rps15 and rbcL had the positive selection sites. Besides, the analysis of mVISTA and sliding windows got a lot of hotspots such as trnH/psbA, rps16/trnQ, trnS/trnG, trnG/trnR and rpl32/trnL, which could be utilized as potential markers for the species identification and phylogeny reconstruction within Ulmus in the further studies. Moreover, the evolutionary tree of Ulmaceae species based on common protein genes, whole cp genome sequences and common genes in IR region of the 23 Ulmaceae species were constructed using the ML method. The results showed that these Ulmaceae species were divided into two branches, one that included Ulmus, Zelkova and Hemiptelea, among which Hemiptelea was the first to differentiate and one that included Celtis, Trema, Pteroceltis, Gironniera and Aphananthe. Besides, these variations found in this study could be used for the classification, identification and phylogenetic study of Ulmus species. Our study provided important genetic information to support further investigations into the phylogenetic development and adaptive evolution of Ulmus and Ulmaceae species.

Project description:BackgroundUlmus mianzhuensis is an endemic tree species in China with high ornamental and economic value. Currently, little is known regarding its genomic architecture, phylogenetic position, or adaptive evolution. Here, we sequenced the complete chloroplast genome (cp genome) of U. mianzhuensis and further compared the variations in gene organization and structure within Ulmus species to define their genomic evolution, then reconstructed the phylogenomic relationship of 31 related Ulmus species to explore the systematic position of U. mianzhuensis and the utility of cp genome for resolving phylogenetics among Ulmus species.ResultsOur results revealed that all the Ulmus species exhibited a typical quadripartite structure, with a large single copy (LSC) region of 87,170 - 88,408 bp, a small single copy (SSC) region of 18,650 - 19,038 bp and an inverted repeat (IR) region of 26,288 - 26,546 bp. Within Ulmus species, gene structure and content of cp genomes were highly conserved, although slight variations were found in the boundary of SC/IR regions. Moreover, genome-wide sliding window analysis uncovered the variability of ndhC-trnV-UAC, ndhF-rpl32, and psbI-trnS-GCU were higher among 31 Ulmus that may be useful for the population genetics and potential DNA barcodes. Two genes (rps15 and atpF) were further detected under a positive selection of Ulmus species. Comparative phylogenetic analysis based on the cp genome and protein-coding genes revealed consistent topology that U. mianzhuensis is a sister group to U. parvifolia (sect. Microptelea) with a relatively low-level nucleotide variation of the cp genome. Additionally, our analyses also found that the traditional taxonomic system of five sections in Ulmus is not supported by the current phylogenomic topology with a nested evolutionary relationship between sections.ConclusionsFeatures of the cp genome length, GC content, organization, and gene order were highly conserved within Ulmus. Furthermore, molecular evidence from the low variation of the cp genome suggested that U. mianzhuensis should be merged into U. parvifolia and regarded as a subspecies of U. parvifolia. Overall, we demonstrated that the cp genome provides valuable information for understanding the genetic variation and phylogenetic relationship in Ulmus.

Dataset Information

The complete chloroplast genome of Celtis julianae (Ulmaceae).

Publications

The complete chloroplast genome of <i>Celtis julianae</i> (Ulmaceae).

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