Ca²⁺-Dependent Glucose Transport in Skeletal Muscle by Diphlorethohydroxycarmalol, an Alga Phlorotannin: In Vitro and In Vivo Study.

ABSTRACT: Diphlorethohydroxycarmalol (DPHC), a type of phlorotannin isolated from the marine alga Ishige okamurae, reportedly alleviates impaired glucose tolerance. However, the molecular mechanisms of DPHC regulatory activity and by which it exerts potential beneficial effects on glucose transport into skeletal myotubes to control glucose homeostasis remain largely unexplored. The aim of this study was to evaluate the effect of DPHC on cytosolic Ca²⁺ levels and its correlation with blood glucose transport in skeletal myotubes in vitro and in vivo. Cytosolic Ca²⁺ levels upon DPHC treatment were evaluated in skeletal myotubes and zebrafish larvae by Ca²⁺ imaging using Fluo-4. We investigated the effect of DPHC on the blood glucose level and glucos

SUBMITTER: Yang HW 

PROVIDER: S-EPMC7889350 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature