Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology.

ABSTRACT: Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly of complex multipart constructs from reusable basic DNA parts in a rapid, reliable and automation-friendly way. Many such toolkits are available, with varying degrees of compatibility, most of which are aimed at specific host organisms. Here, we present a vector design which allows simple vector modification by using modular cloning to assemble and add new functions in secondary sites flanking the main insertion site (used for conventional modular cloning). Assembly in all sites is compatible with the PhytoBricks standard, and vectors are compatible with the Standard European Vector

SUBMITTER: Valenzuela-Ortega M 

PROVIDER: S-EPMC7889407 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature